6ZFG

14-3-3 zeta chimera with 18E6 and fusicoccin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	73.230, 76.100, 88.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.400 - 1.850
R-factor	0.1921
Rwork	0.191
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o02
RMSD bond length	0.014
RMSD bond angle	0.949
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.400	1.900
High resolution limit [Å]	1.850	1.850
Rmeas	0.163	2.160
Number of reflections	41927	3038
<I/σ(I)>	12.36	1.4
Completeness [%]	97.1	96.5
Redundancy	13.3	13.6
CC(1/2)	0.998	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	19% PEG4000, 0.1M cacodylate, 20%glycerol as a cryoprotectant