6ZFC
Fucose-binding lectin from Burkholderia ambifaria (BamBL) in complex with a fucosyl derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 109.092, 49.163, 115.098 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 45.210 - 1.650 |
| R-factor | 0.1782 |
| Rwork | 0.177 |
| R-free | 0.20850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zw0 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.687 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.250 | 45.210 | 1.680 |
| High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
| Rmerge | 0.088 | 0.034 | 1.260 |
| Rmeas | 0.095 | 0.037 | 1.360 |
| Rpim | 0.036 | 0.014 | 0.509 |
| Total number of observations | 3153 | 24707 | |
| Number of reflections | 72307 | 488 | 3494 |
| <I/σ(I)> | 11.8 | 33.8 | 1.5 |
| Completeness [%] | 98.1 | 99.1 | 96.5 |
| Redundancy | 6.9 | 6.5 | 7.1 |
| CC(1/2) | 0.999 | 0.999 | 0.627 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 200 mM trisodium citrate, 100 mM sodium acetate pH 5.0 and 24% PEG 8000, 1% DMSO |
