6ZEJ

Structure of PP1-Phactr1 chimera [PP1(7-304) + linker (SGSGS) + Phactr1(526-580)]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.911
Spacegroup name	P 65
Unit cell lengths	137.370, 137.370, 238.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.680 - 1.780
R-factor	0.2433
Rwork	0.242
R-free	0.27140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mov
RMSD bond length	0.011
RMSD bond angle	0.840
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.680	1.840
High resolution limit [Å]	1.780	1.780
Rmerge	0.170	3.180
Rmeas	0.180	3.260
Rpim	0.040	0.700
Number of reflections	241814	24087
<I/σ(I)>	12.9	1.1
Completeness [%]	99.7	99.6
Redundancy	20.7	21
CC(1/2)	1.000	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	293	20% PEG 3350, 0.2M Potassium Citrate