6ZDW

Crystal structure of the ribonuclease core of R3B2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	75.910, 80.759, 83.783
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.953 - 1.650
Rwork	0.162
R-free	0.18240
Structure solution method	SIRAS
RMSD bond length	0.005
RMSD bond angle	1.283
Data reduction software	Aimless (1.0.2)
Data scaling software	Aimless (1.0.2)
Phasing software	AutoSol (1.17.1)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.950	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.044
Rmeas	0.048
Rpim	0.018
Number of reflections	62534	2937
<I/σ(I)>	21.8
Completeness [%]	99.8	96.9
Redundancy	12.4	9.1
CC(1/2)	0.999	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	100 mM Hepes/NaOH pH 7.0, 200 mM L-proline and 12% (w/v) PEG3350
1	VAPOR DIFFUSION		291.15	100 mM Hepes/NaOH pH 7.0, 200 mM L-proline and 12% (w/v) PEG3350