6ZDA
Crystal structure of YTHDC1 M438A mutant complex with m6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-17 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.090, 103.330, 42.350 |
| Unit cell angles | 90.00, 105.93, 90.00 |
Refinement procedure
| Resolution | 40.720 - 1.300 |
| R-factor | 0.2049 |
| Rwork | 0.204 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.720 | 1.380 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 80684 | 12941 |
| <I/σ(I)> | 15.78 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.48 | |
| CC(1/2) | 0.999 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5 |
