6ZD1

Structure of apo telomerase from Candida Tropicalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978530
Spacegroup name	P 41 21 2
Unit cell lengths	65.798, 65.798, 387.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.780 - 2.470
R-factor	0.248
Rwork	0.246
R-free	0.28410
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.718
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.780	2.555
High resolution limit [Å]	2.467	2.467
Rmerge	0.150	1.652
Rmeas	0.163	1.804
Rpim	0.062	0.507
Number of reflections	54095	996
<I/σ(I)>	10.47	1.4
Completeness [%]	85.1	45.9
Redundancy	6.8	12.6
CC(1/2)	0.991	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.1	298	0.2 M sodium citrate tribasic dihydrate 0.1 M Bis-Tris propane (pH 6.1) 15% PEG3350