6ZC7
Small-molecule inhibitors of the PDZ domain of Dishevelled proteins interrupt Wnt signalling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 61 |
| Unit cell lengths | 85.339, 85.339, 58.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.550 - 1.480 |
| R-factor | 0.1547 |
| Rwork | 0.153 |
| R-free | 0.18090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f0a |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.045 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.550 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmeas | 0.007 | 0.081 |
| Number of reflections | 40755 | 3012 |
| <I/σ(I)> | 18.4 | 3.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.26 M sodium phosphate, 0.14 M potassium phosphate |
