6ZC5
Human Adenovirus serotype D10 FiberKnob protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 31 |
| Unit cell lengths | 101.357, 101.357, 326.717 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 108.910 - 2.500 |
| R-factor | 0.2206 |
| Rwork | 0.219 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6stt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.576 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 108.910 | 108.910 | 2.540 |
| High resolution limit [Å] | 2.500 | 13.690 | 2.500 |
| Rmerge | 0.147 | 0.069 | 2.766 |
| Rmeas | 0.162 | 0.076 | 3.030 |
| Rpim | 0.067 | 0.033 | 1.231 |
| Total number of observations | 741038 | 4315 | 38530 |
| Number of reflections | 128287 | 797 | 6440 |
| <I/σ(I)> | 6.7 | 22.4 | 0.8 |
| Completeness [%] | 98.7 | 99.1 | 99.3 |
| Redundancy | 5.8 | 5.4 | 6 |
| CC(1/2) | 0.996 | 0.993 | 0.732 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2 M CaCl2, 0.1 MES, 20% w/v PEG 6000 |
