Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 73.150, 73.150, 131.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.151 - 1.850 |
| Rwork | 0.185 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yz5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.682 |
| Data reduction software | XSCALE |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.151 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.086 | 2.050 |
| Rpim | 0.028 | 0.460 |
| Number of reflections | 35506 | 2585 |
| <I/σ(I)> | 18.2 | 1.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 19.7 | 20.9 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Crystals were grown at using the sitting drop vapor diffusion method by mixing 0.2 uL of the 18 mg/mL H11-H4 RBD complex with 0.1 uL of the crystallization buffer containing 0.2 M Sodium acetate trihydrate, 0.1 M MES pH 6.0, 20 % w/v PEG 8000. The crystals grew overnight and were flash cooled in a solution containing the mother liquor with 30 % (v/v) ethylene glycol. |
