6ZB8
Exo-beta-1,3-glucanase from moose rumen microbiome, active site mutant E167Q/E295Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.826561 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.630, 87.790, 70.860 |
Unit cell angles | 90.00, 100.96, 90.00 |
Refinement procedure
Resolution | 29.350 - 1.350 |
R-factor | 0.1529 |
Rwork | 0.153 |
R-free | 0.16250 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | Wild-type structure solved by MR-SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.007 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.350 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 139214 | 10636 |
<I/σ(I)> | 13.2 | 1.1 |
Completeness [%] | 95.2 | 70.8 |
Redundancy | 6.6 | 5 |
CC(1/2) | 0.997 | 0.373 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M TrisHCl pH 8.5, 0.2M MgCl, 30% PEG 4000 |