6ZB1

C-TERMINAL BROMODOMAIN OF HUMAN BRD2 WITH GSK620

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-02
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	52.428, 71.855, 31.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.953 - 1.600
Rwork	0.154
R-free	0.18520
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	in house structure
RMSD bond length	0.005
RMSD bond angle	1.207
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.860	1.680
High resolution limit [Å]	1.600	1.600
Rmerge	0.017	0.058
Number of reflections	1588	1950
<I/σ(I)>	42	12.3
Completeness [%]	95.8	83.2
Redundancy	3.3	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	30% PEG 300, 0.1M MES buffer pH6.5