6Z99

Copper transporter OprC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-18
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	1.35132
Spacegroup name	C 2 2 21
Unit cell lengths	154.644, 195.506, 165.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.180 - 2.680
R-factor	0.2166
Rwork	0.215
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fok
RMSD bond length	0.009
RMSD bond angle	1.121
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.180	2.730
High resolution limit [Å]	2.680	2.680
Rpim	0.056
Number of reflections	70434	6894
<I/σ(I)>	0.996
Completeness [%]	99.9
Redundancy	13.51
CC(1/2)		0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.2 M Choline chloride 0.1 M Tris 12-16 % w/v PEG 2000 MME