6Z7T

Nucleotide-free Myosin-II motor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	56.681, 174.395, 100.252
Unit cell angles	90.00, 106.38, 90.00

Refinement procedure

Resolution	46.360 - 1.880
R-factor	0.1911
Rwork	0.191
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aka
RMSD bond length	0.011
RMSD bond angle	1.211
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.360	1.950
High resolution limit [Å]	1.880	1.880
Number of reflections	148819	14584
<I/σ(I)>	9.46	1.81
Completeness [%]	98.7
Redundancy	5.9
CC(1/2)	0.997	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	100 mM HEPES, pH7.5, 10% PEG8000, 8% Ethylenglycol