6Z7E

Variant Surface Glycoprotein VSGsur mutant H122A soaked in 7.7 mM suramin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	46.936, 71.033, 130.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.160 - 1.663
R-factor	0.1857
Rwork	0.184
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z79
Data scaling software	SCALA
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.160	1.723
High resolution limit [Å]	1.663	1.663
Number of reflections	52136	5028
<I/σ(I)>	19.44	1.9
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	1.000	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	19-24 % PEG 400 100 mM TEA/HCl pH=7.5 10 % (v/v) isopropanol