6Z7C

Variant Surface Glycoprotein VSGsur mutant H122A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-26
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 2
Unit cell lengths	46.945, 70.919, 130.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.250 - 1.640
R-factor	0.1885
Rwork	0.187
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z79
Data scaling software	xia2
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.310	65.380	1.500
High resolution limit [Å]	1.470	3.990	1.470
Rmeas	0.112	0.041	3.118
Rpim	0.031	0.012	0.851
Total number of observations	990624	51716	48830
Number of reflections	75184	4066	3652
<I/σ(I)>	10.9	62.3	0.4
Completeness [%]	100.0	100	100
Redundancy	13.2	12.7	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	19-24 % PEG 400 100 mM TEA/HCl pH=7.5 10 % (v/v) isopropanol