6Z5Y

Structure of a novel LPMO from Phytophthora infestans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.916
Spacegroup name	P 1
Unit cell lengths	36.293, 45.458, 53.687
Unit cell angles	83.91, 71.85, 83.78

Refinement procedure

Resolution	28.790 - 1.010
R-factor	0.1441
Rwork	0.143
R-free	0.16400
Structure solution method	AB INITIO PHASING
RMSD bond length	0.015
RMSD bond angle	1.957
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	Fragon
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.790	28.770	1.030
High resolution limit [Å]	1.010	5.530	1.010
Rmerge	0.048	0.035	0.680
Rmeas	0.057	0.041	0.856
Rpim	0.030	0.021	0.509
Number of reflections	156702	992	6765
<I/σ(I)>	8.9
Completeness [%]	92.4	95.9	80.9
Redundancy	3.3	3.8	2.7
CC(1/2)	0.998	0.997	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20mM Tris pH 7.0, 22% Polyethylene Glycol (PEG) 3350