6Z2L
Structure of Plasmodium falciparum P113 bound to antibody P3.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 96.921, 96.921, 177.898 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.920 - 1.950 |
R-factor | 0.2058 |
Rwork | 0.205 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u0r |
RMSD bond length | 0.013 |
RMSD bond angle | 1.130 |
Data reduction software | autoPROC |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.920 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rpim | 0.042 | 1.422 |
Number of reflections | 62581 | 4310 |
<I/σ(I)> | 16.6 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.2 | 26.2 |
CC(1/2) | 0.994 | 0.571 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M NH4SO4, 0.1M MES pH=6.5, 20% w/v PEG8000 |