6Z27
Photosynthetic Reaction Center From Rhodobacter Sphaeroides strain RV LCP crystallization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.8610 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 99.914, 99.914, 234.047 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.860 - 2.100 |
R-factor | 0.2092 |
Rwork | 0.209 |
R-free | 0.23570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v3y |
RMSD bond length | 0.008 |
RMSD bond angle | 1.031 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (1.18_3861) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.075 | |
Number of reflections | 72564 | 5862 |
<I/σ(I)> | 15.94 | 1.14 |
Completeness [%] | 99.9 | |
Redundancy | 11.06 | |
CC(1/2) | 0.990 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 289 | 1-mono-oleyl-rac-glycerol (MO), 1,2,3-heptanetriol, Jeffamine M600, N,N-dimethyldodecylamine-N-oxide (LDAO) |