6Z1V

Structure of the EC2 domain of CD9 in complex with nanobody 4E8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 21 21 2
Unit cell lengths	61.521, 89.446, 35.684
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.720 - 1.330
R-factor	0.1531
Rwork	0.151
R-free	0.18980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Structure of CD9EC2 bound to nanobody 4C8
RMSD bond length	0.008
RMSD bond angle	0.941
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.720	1.440
High resolution limit [Å]	1.330	1.330
Rmerge	0.040	0.980
Rpim	0.020	0.600
Number of reflections	32044	1603
<I/σ(I)>	17.6	1.5
Completeness [%]	92.0	68.9
Redundancy	6.4	6.1
CC(1/2)	1.000	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.2 M sodium acetate, 0.1 M Tris pH 8.0, 30% (w/v) PEG 4,000 cryoprotected with reservoir solution supplemented with 20% (v/v) ethylene glycol.