6Z1H
Ancestral glycosidase (family 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.263, 80.668, 97.808 |
| Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
| Resolution | 48.150 - 2.500 |
| R-factor | 0.1964 |
| Rwork | 0.194 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j78 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.663 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.150 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 27987 | 3063 |
| <I/σ(I)> | 10.9 | 1.7 |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 7.4 | 7.5 |
| CC(1/2) | 0.999 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | HR1 (#C41): 0.1 M HEPES Na pH 7.5, 10% v/v 2-Propanol, 20% w/v PEG 4,000 |
