6Z1H

Ancestral glycosidase (family 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	52.263, 80.668, 97.808
Unit cell angles	90.00, 100.06, 90.00

Refinement procedure

Resolution	48.150 - 2.500
R-factor	0.1964
Rwork	0.194
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j78
RMSD bond length	0.003
RMSD bond angle	0.663
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.150	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	27987	3063
<I/σ(I)>	10.9	1.7
Completeness [%]	99.7	97.4
Redundancy	7.4	7.5
CC(1/2)	0.999	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	HR1 (#C41): 0.1 M HEPES Na pH 7.5, 10% v/v 2-Propanol, 20% w/v PEG 4,000