6Z0Z

Human wtSTING in complex with 3',3'-c-(2'FdAMP-2'FdAMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 43 21 2
Unit cell lengths	67.174, 67.174, 136.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.797 - 2.499
R-factor	0.1929
Rwork	0.191
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ksy
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	2.588
High resolution limit [Å]	2.499	2.499
Rmerge	0.132	0.905
Rmeas	0.141	0.964
Rpim	0.050	0.329
Number of reflections	11367	1099
<I/σ(I)>	11.76	2.16
Completeness [%]	99.8
Redundancy	7.8
CC(1/2)	0.997	0.776

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Lithium acetate, 20% (w/v) PEG 3350