6Z0O

Structure of Affimer-NP bound to Crimean-Congo Haemorrhagic Fever Virus Nucleocapsid Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	90.330, 73.980, 95.764
Unit cell angles	90.00, 100.92, 90.00

Refinement procedure

Resolution	88.694 - 2.597
R-factor	0.2275
Rwork	0.225
R-free	0.27220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4akl 4n6u
RMSD bond length	0.003
RMSD bond angle	0.748
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.000	2.710
High resolution limit [Å]	2.597	2.600
Number of reflections	26028	126
<I/σ(I)>	10.3
Completeness [%]	67.1
Redundancy	6.1
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	ammonium acetate, sodium acetate, PEG 4000