6Z0J
Crystal structure of laccase from Pediococcus acidilactici Pa5930 (Tris-HCl pH 8.5)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.797, 94.294, 97.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.800 - 2.500 |
| R-factor | 0.2227 |
| Rwork | 0.220 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zdw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.264 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.8) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 94.290 | 94.290 | 2.600 |
| High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
| Rmerge | 0.148 | 0.063 | 0.904 |
| Rmeas | 0.160 | 0.069 | 0.970 |
| Rpim | 0.058 | 0.027 | 0.350 |
| Total number of observations | 124640 | 2648 | 14100 |
| Number of reflections | 17180 | 437 | 1879 |
| <I/σ(I)> | 7.9 | 17.3 | 2.2 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 7.3 | 6.1 | 7.5 |
| CC(1/2) | 0.995 | 0.995 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 30% PEG1000 and 0.1M Tris-HCl pH 8.5 |
