6Z06
Crystal structure of Puumala virus Gc in complex with Fab 4G2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.968590 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.213, 50.449, 124.446 |
| Unit cell angles | 90.00, 107.66, 90.00 |
Refinement procedure
| Resolution | 92.633 - 3.500 |
| R-factor | 0.2196 |
| Rwork | 0.217 |
| R-free | 0.26390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j81 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.14) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 92.633 | 92.633 | 3.550 |
| High resolution limit [Å] | 3.500 | 9.470 | 3.500 |
| Rmeas | 0.437 | 0.173 | 1.489 |
| Rpim | 0.140 | 0.057 | 0.511 |
| Number of reflections | 14920 | 818 | 702 |
| <I/σ(I)> | 4.6 | 8.8 | 1.5 |
| Completeness [%] | 99.8 | 100 | 93 |
| Redundancy | 9.6 | 8.8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 20% PEG6000, 1 M LiCl, 0.1 M MES pH 6, 6% 2-methyl-2,4-pentanediol |
