6YZZ
Arabidopsis thaliana Naa50 in complex with AcCoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0781 |
Spacegroup name | I 2 3 |
Unit cell lengths | 100.164, 100.164, 100.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.890 - 1.790 |
R-factor | 0.1713 |
Rwork | 0.170 |
R-free | 0.19700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ob0 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.921 |
Data reduction software | XDS (VERSION Nov 11, 2017 BUILT=20171111) |
Data scaling software | Aimless (7.0.073) |
Phasing software | PHASER (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.890 | 1.820 |
High resolution limit [Å] | 1.790 | 1.790 |
Rpim | 0.019 | 0.752 |
Number of reflections | 16046 | 914 |
<I/σ(I)> | 21.2 | 1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | 7.6 |
CC(1/2) | 1.000 | 0.616 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 34 mg mL-1 protein (20 mM HEPES pH 8.0, 250 mM NaCl) mixed with AcCoA (5.4 mM); drops (600 nL): 1:1 mix of (20 mM HEPES pH 8.0, 250 mM NaCl) and (0.9 M sodium citrate, 0.1 M sodium cacodylate pH 6.5). Growth after 19 h, 20 % glycerol (v/v) as cryo protection |