6YZ2

Full length Open-form Sodium Channel NavMs F208L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9797
Spacegroup name	I 4 2 2
Unit cell lengths	108.940, 108.940, 209.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	104.680 - 2.200
R-factor	0.2438
Rwork	0.243
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvx
RMSD bond length	0.008
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (19-MAR-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.680	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	32388	2771
<I/σ(I)>	17.4	2.2
Completeness [%]	100.0
Redundancy	21.2
CC(1/2)	0.999	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M lithium sulphate, 0.1M HEPES, pH 7, and 40% v/v PEG200.