6YZ0

Full length Open-form Sodium Channel NavMs F208L in complex with Cannabidiol (CBD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-09-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9797
Spacegroup name	I 4 2 2
Unit cell lengths	110.328, 110.328, 207.876
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	103.940 - 2.300
R-factor	0.2479
Rwork	0.247
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvx
RMSD bond length	0.008
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (19-MAR-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.940	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	29849	2770
<I/σ(I)>	17.3	1
Completeness [%]	100.0
Redundancy	21
CC(1/2)	0.999	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1M lithium sulphate, 0.1M HEPES, pH 7, and 40% v/v PEG200.