6YYI

Crystal structure of beta-D-xylosidase from Dictyoglomus thermophilum bound to beta-D-xylopyranose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99
Spacegroup name	P 31 2 1
Unit cell lengths	160.950, 160.950, 103.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.570 - 2.670
R-factor	0.1799
Rwork	0.177
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1px8
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.570	48.570	2.840
High resolution limit [Å]	2.670	7.930	2.670
Rmerge	0.172	0.043	1.138
Rmeas	0.176	0.044	1.167
Total number of observations	911219
Number of reflections	43883	1798	6911
<I/σ(I)>	17.17	62.62	2.71
Completeness [%]	99.8	99.5	98.6
Redundancy	20.765	19.758	20.285
CC(1/2)	0.999	1.000	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	0.1 M Ammonium sulfate 10% PEG 4000 0,1M Sodium citrate pH 5.6