6YYE

TREM2 extracellular domain (19-131) in complex with single-chain variable fragment (scFv-2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-17
Detector	RAYONIX MX-300
Wavelength(s)	0.9763
Spacegroup name	I 2 2 2
Unit cell lengths	113.942, 126.201, 225.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	110.070 - 3.360
R-factor	0.2998
Rwork	0.298
R-free	0.32720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cy6
RMSD bond length	0.003
RMSD bond angle	0.535
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.070	3.679
High resolution limit [Å]	3.356	3.356
Number of reflections	16278	814
<I/σ(I)>	7.4
Completeness [%]	92.0
Redundancy	13.4
CC(1/2)	0.998	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.015M nickel chloride,0.1M tris pH 8.5,2.2M ammonium acetate