6YXW

Affimer K3 - KRAS protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9660
Spacegroup name	P 1 21 1
Unit cell lengths	73.126, 39.490, 113.212
Unit cell angles	90.00, 106.91, 90.00

Refinement procedure

Resolution	54.160 - 2.060
R-factor	0.2385
Rwork	0.236
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4obe 4n6t
RMSD bond length	0.006
RMSD bond angle	1.020
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.160	2.110
High resolution limit [Å]	2.060	2.060
Rmerge	0.089	0.393
Rpim	0.063	0.465
Number of reflections	38740	2830
<I/σ(I)>	6.2
Completeness [%]	99.7	99.9
Redundancy	2.9	3
CC(1/2)	0.978	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	2M ammonium sulfate, 0.2M potassium sodium tartrate, 0.1M tri-sodium citrate