6YXP

Higher resolution structure of the N-terminal module of the human SWI/SNF-subunit BAF155/SMARCC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.939273
Spacegroup name	P 1 21 1
Unit cell lengths	43.900, 138.322, 56.510
Unit cell angles	90.00, 110.31, 90.00

Refinement procedure

Resolution	42.066 - 1.600
R-factor	0.174
Rwork	0.173
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yxo
RMSD bond length	0.006
RMSD bond angle	0.800
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.490	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.053	0.611
Rmeas	0.057	0.661
Rpim	0.022	0.248
Number of reflections	82320	11930
<I/σ(I)>	15.1	2.7
Completeness [%]	99.1	98.2
Redundancy	6.8	6.9
CC(1/2)	0.999	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	290	0.2 M Magnesium formate (pH 5.9), 20% PEG 3350. Protein at 16 mg/ml.