6YXD

Room temperature structure of human adiponectin receptor 2 (ADIPOR2) at 2.9 A resolution determined by Serial Crystallography (SSX) using CrystalDirect

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-07-01
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.988
Spacegroup name	P 21 2 21
Unit cell lengths	75.460, 100.760, 114.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.900
R-factor	0.2013
Rwork	0.199
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lwy
RMSD bond length	0.006
RMSD bond angle	0.820
Data reduction software	CrystFEL (0.8.0)
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.980
High resolution limit [Å]	2.900	2.900
Number of reflections	15292	1971
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	130.57
CC(1/2)	0.980	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	CrystalDirect-2 plates (MiTeGen) in 30 or 50 nL bolus overlaid with 600 nl precipitant solution. 42.3% PEG 400, 110 mM potassium citrate, 100 mM HEPES pH 7.0 for ADIPOR2.