6YX7

The high resolution structure of allophycocyanin from cyanobacterium Nostoc sp. WR13, the P21212 crystal form.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97932
Spacegroup name	P 21 21 2
Unit cell lengths	123.695, 177.726, 106.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.372 - 1.419
Rwork	0.126
R-free	0.17510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b33
RMSD bond length	0.009
RMSD bond angle	1.517
Data reduction software	XDS (June 26, 2018 (20180409))
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.372	1.503
High resolution limit [Å]	1.419	1.419
Rmerge	0.077	1.220
Rmeas	0.082	1.313
Rpim	0.027	0.478
Number of reflections	383047	19080
<I/σ(I)>	13.8	1.7
Completeness [%]	96.1	61.3
Redundancy	8.7	7.2
CC(1/2)	0.998	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Morpheus screen condition H12: Precipitant mix 4: 25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350; Buffer system 3: 1.0M Tris (base); bicine, pH 8.5 Additives: amino acids: 0.2M Gly, DL-Ala, DL-Ser, DL-Glu, DL-Lys