6YX6

Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148K variant (no morpholine)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9737
Spacegroup name	P 21 21 2
Unit cell lengths	57.772, 110.839, 39.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.900 - 1.500
R-factor	0.1754
Rwork	0.174
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yx4
RMSD bond length	0.005
RMSD bond angle	1.329
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.993	1.539
High resolution limit [Å]	1.500	1.500
Number of reflections	35712	1786
<I/σ(I)>	14.8	2.9
Completeness [%]	86.6	59.9
Redundancy	5.6	5.6
CC(1/2)	0.999	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.5M Ammonium Sulfate, 0.1M tri-Sodium citrate, pH 6.5, 1M Lithium sulfate