6YWI
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148E variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9720 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 53.550, 109.840, 38.951 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.180 - 1.130 |
R-factor | 0.1728 |
Rwork | 0.172 |
R-free | 0.18170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rhf |
RMSD bond length | 0.008 |
RMSD bond angle | 1.422 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 54.920 | 54.920 | 1.150 |
High resolution limit [Å] | 1.130 | 6.190 | 1.130 |
Rmerge | 0.069 | 0.067 | 0.675 |
Rmeas | 0.075 | 0.074 | 0.741 |
Rpim | 0.029 | 0.030 | 0.300 |
Total number of observations | 540189 | 3821 | 24570 |
Number of reflections | 86013 | 634 | 4192 |
<I/σ(I)> | 12.3 | 27 | 2.4 |
Completeness [%] | 99.1 | 99.6 | 98.5 |
Redundancy | 6.3 | 6 | 5.9 |
CC(1/2) | 0.997 | 0.990 | 0.801 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M glutamic acid, 0.1M alanine, 0.1M glycine, 0.1M lysine, 0.1M serine, 0.1M MES, 0.1M Imid, pH 6.5, 20%PEG500, 10% PEG20000 |