6YWB

Human wtSTING in complex with 3',3'-c-[2'FdAMP-2'FdAM(PS)]

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-09
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 41 21 2
Unit cell lengths	110.906, 110.906, 35.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.070 - 2.503
R-factor	0.2117
Rwork	0.209
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ksy
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.070	2.593
High resolution limit [Å]	2.503	2.503
Rmerge	0.162	1.031
Rpim	0.060	0.371
Number of reflections	8087	769
<I/σ(I)>	10.69
Completeness [%]	99.5
Redundancy	8.2
CC(1/2)	0.997	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.0 M Sodium/Potassium tartrate, 0.2 M Lithium sulfate, 0.1 M Tris pH 7.0