6YVP

Human histidine triad nucleotide-binding protein 2 (hHINT2) complexed with dGMP and refined to 2.77 A

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	71.649, 36.531, 86.887
Unit cell angles	90.00, 103.03, 90.00

Refinement procedure

Resolution	42.320 - 2.770
Rwork	0.208
R-free	0.30770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6YQD:B
RMSD bond length	0.005
RMSD bond angle	1.491
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.7.02)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.320	2.920
High resolution limit [Å]	2.770	2.770
Rmerge	0.198	0.668
Rmeas	0.266	0.894
Rpim	0.176	0.589
Number of reflections	5698	814
<I/σ(I)>	5.5	2
Completeness [%]	99.1	99.1
Redundancy	3.8	3.9
CC(1/2)	0.985	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281	15 % (w/v) PEG6000, 5 % (v/v) glycerol