6YV7
Mannosyltransferase PcManGT from Pyrobaculum calidifontis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.06884 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.449, 107.608, 163.572 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.079 - 2.700 |
R-factor | 0.2505 |
Rwork | 0.247 |
R-free | 0.29020 |
Structure solution method | SIRAS |
RMSD bond length | 0.014 |
RMSD bond angle | 1.878 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.080 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 48112 | 4916 |
<I/σ(I)> | 12.2 | 2.5 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7 | |
CC(1/2) | 0.997 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 mM HEPES (pH 7.5), 20% PEG 4000, 10% isopropanol, 10 mM MnCl2 and 2% 1,2,3-heptanetriol |