Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000031 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 125.479, 125.479, 124.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.360 - 2.750 |
| Rwork | 0.263 |
| R-free | 0.28380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pe2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.360 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rpim | 0.045 | 0.316 |
| Number of reflections | 26481 | 3806 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.6 | |
| CC(1/2) | 0.998 | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5 0.2 ammonia sulphate 30% PEG 5000 MME 10 mg/mL protein |
