6YRQ

Crystal structure of the tetramerization domain of the glycoprotein Gn (Andes virus) at pH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	90.636, 123.056, 90.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.318 - 1.902
R-factor	0.1896
Rwork	0.187
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yrb
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.14rc3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.320	45.320	1.940
High resolution limit [Å]	1.900	9.120	1.900
Rmerge	0.116	0.058	3.012
Rmeas	0.120	0.061	3.134
Rpim	0.033	0.018	0.854
Total number of observations	542782	5089	31473
Number of reflections	39873	414	2451
<I/σ(I)>	11.1	35.9	0.9
Completeness [%]	99.8	99.3	97
Redundancy	13.6	12.3	12.8
CC(1/2)	0.998	0.998	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M CaCl2, 0.1M Na-acetate 4.6, 30% MPD