6YRQ
Crystal structure of the tetramerization domain of the glycoprotein Gn (Andes virus) at pH 4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.636, 123.056, 90.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.318 - 1.902 |
| R-factor | 0.1896 |
| Rwork | 0.187 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yrb |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14rc3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.320 | 45.320 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.120 | 1.900 |
| Rmerge | 0.116 | 0.058 | 3.012 |
| Rmeas | 0.120 | 0.061 | 3.134 |
| Rpim | 0.033 | 0.018 | 0.854 |
| Total number of observations | 542782 | 5089 | 31473 |
| Number of reflections | 39873 | 414 | 2451 |
| <I/σ(I)> | 11.1 | 35.9 | 0.9 |
| Completeness [%] | 99.8 | 99.3 | 97 |
| Redundancy | 13.6 | 12.3 | 12.8 |
| CC(1/2) | 0.998 | 0.998 | 0.537 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M CaCl2, 0.1M Na-acetate 4.6, 30% MPD |
