6YRO
Streptococcus suis SadP mutant - N285D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.132, 80.799, 130.740 |
| Unit cell angles | 90.00, 113.13, 90.00 |
Refinement procedure
| Resolution | 45.680 - 2.050 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bob |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.932 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.300 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmeas | 0.163 | 0.717 |
| Number of reflections | 71993 | 3213 |
| <I/σ(I)> | 6.2 | 1.5 |
| Completeness [%] | 94.9 | 69 |
| Redundancy | 3.7 | 2.9 |
| CC(1/2) | 0.989 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.3-1.5 M sodium citrate tribasic, 0.1 M sodium cacodylate |
