6YR8

Affimer K6 - KRAS protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	I 41
Unit cell lengths	71.574, 71.574, 144.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.130 - 1.900
R-factor	0.2127
Rwork	0.211
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4obe 4n6t
RMSD bond length	0.009
RMSD bond angle	1.290
Data scaling software	xia2 (0.5.769)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.130	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.087	2.690
Rpim	0.025	0.834
Number of reflections	28572	2116
<I/σ(I)>	13.3	0.6
Completeness [%]	100.0	100
Redundancy	13.3	11.4
CC(1/2)	1.000	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	0.1M sodium acetate, 25% w/v PEG 4000, 0.2M ammonium sulfate, 5% MPD