6YQM
Human histidine triad nucleotide-binding protein 1 (hHINT1) complexed with dGMP and refined to 1.02 A
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-07 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.658, 46.434, 64.102 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 39.960 - 1.020 |
| Rwork | 0.112 |
| R-free | 0.13560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tw2 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.403 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP (11.7.02) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.963 | 1.040 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.042 | 0.466 |
| Rmeas | 0.056 | 0.630 |
| Rpim | 0.037 | 0.422 |
| Number of reflections | 116020 | 5440 |
| <I/σ(I)> | 14.1 | 2.1 |
| Completeness [%] | 99.1 | 93.6 |
| Redundancy | 3.8 | 3.3 |
| CC(1/2) | 0.999 | 0.785 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 281 | 16 % (w/v) PEG 4000, 0.1 M sodium cacodylate |
