6YQF

Crystal structure of the SYCE2-TEX12 delta-Ctip complex in a 4:4 assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9688
Spacegroup name	P 21 21 2
Unit cell lengths	42.670, 59.680, 156.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.450 - 3.330
R-factor	0.3286
Rwork	0.327
R-free	0.36720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r17
RMSD bond length	0.001
RMSD bond angle	0.272
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.452	3.594
High resolution limit [Å]	3.327	3.327
Rmeas	0.246	2.445
Rpim	0.098	0.906
Number of reflections	4098	342
<I/σ(I)>	4.7	1
Completeness [%]	64.8	27.1
Redundancy	6.5	7.2
CC(1/2)	0.995	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate, 65% MPD