6YP9

Rabbit muscle actin in complex with ADF-H and ATP-ATTO-488

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	50.552, 69.017, 147.948
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.550 - 2.564
R-factor	0.1767
Rwork	0.174
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3daw
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS (BUILT=20200131 (Jan 31, 2020))
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.5.6)
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.546	2.608
High resolution limit [Å]	2.564	2.564
Rmerge	0.146	0.885
Rmeas	0.161	0.969
Rpim	0.065	0.390
Number of reflections	17247	822
<I/σ(I)>	10.3	2.3
Completeness [%]	99.8	100
Redundancy	6	6.1
CC(1/2)	0.996	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M sodium cacodylate (pH 6.0) and 15% (w/v) PEG 4000