6YOP
Structure of SAMM50 LIR bound to GABARAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 101.323, 101.323, 101.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.660 - 1.100 |
| R-factor | 0.1392 |
| Rwork | 0.138 |
| R-free | 0.15880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnu |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.683 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.660 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.080 | 2.640 |
| Rpim | 0.010 | 0.290 |
| Number of reflections | 5665274 | 567531 |
| <I/σ(I)> | 27.3 | 0.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 81.1 | 81.8 |
| CC(1/2) | 1.000 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% v/v isopropanol, 0.1 M Na Acetate pH 5.5 |
