6YON
Crystal structure of Endoglucanase S127C/A165C from Penicillium verruculosum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2019-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978565037251 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.500, 85.690, 91.150 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 49.340 - 2.600 |
| R-factor | 0.23923 |
| Rwork | 0.236 |
| R-free | 0.29197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i6s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.608 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 48284 | 3354 |
| <I/σ(I)> | 5.15 | |
| Completeness [%] | 99.2 | |
| Redundancy | 4.08 | |
| CC(1/2) | 0.994 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285 | PEG 6000,Bis-Tris-Propane, MPD, tert-butanol |
