6YO9
Product bound structure of the Ectoine utilization protein EutD (DoeA) from Halomonas elongata
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 157.073, 157.073, 124.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.390 - 2.400 |
Rwork | 0.184 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6twj |
RMSD bond length | 0.008 |
RMSD bond angle | 0.959 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.484 |
High resolution limit [Å] | 2.399 | 2.399 |
Rmerge | 0.190 | |
Rpim | 0.051 | |
Number of reflections | 60665 | 5767 |
<I/σ(I)> | 12.02 | |
Completeness [%] | 99.1 | |
Redundancy | 14.7 | |
CC(1/2) | 0.998 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M trisodium citrate, 20 % (w/v) PEG 3350 |