6YNF
GAPDH purified from the supernatant of HEK293F cells: crystal form 3 of 4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-22 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.136, 111.429, 135.943 |
| Unit cell angles | 90.00, 96.02, 90.00 |
Refinement procedure
| Resolution | 135.180 - 2.394 |
| R-factor | 0.1808 |
| Rwork | 0.179 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ynd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.180 | 2.740 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.270 | 1.280 |
| Rmeas | 0.290 | 1.380 |
| Number of reflections | 59243 | 2962 |
| <I/σ(I)> | 6.5 | 1.5 |
| Completeness [%] | 58.2 | 8.7 |
| Redundancy | 7.1 | 7 |
| CC(1/2) | 0.986 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Condition F4 of MORPHEUS screen. 0.12M monosaccharides solution, 0.1M MORPHEUS Buffer System 3, 30% v/v MORPHEUS Precipitant Mix 1 |
