6YN8
Human MALT1(334-719) in complex with a tetrazole containing compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99990 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 101.293, 106.356, 106.455 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.400 - 3.052 |
| R-factor | 0.2143 |
| Rwork | 0.213 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v55 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4 (13-Dec-2018)) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (6-FEB-2020)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.400 | 60.400 | 3.062 |
| High resolution limit [Å] | 3.052 | 14.105 | 3.052 |
| Rmerge | 0.078 | 0.028 | 0.935 |
| Rmeas | 0.085 | 0.032 | 1.015 |
| Rpim | 0.033 | 0.014 | 0.394 |
| Total number of observations | 72615 | 713 | 762 |
| Number of reflections | 11243 | 140 | 116 |
| <I/σ(I)> | 15.8 | 40.4 | 2.2 |
| Completeness [%] | 99.8 | 98.6 | 100 |
| Redundancy | 6.5 | 5.1 | 6.6 |
| CC(1/2) | 0.999 | 0.999 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 294 | 1.8 M Sodium/Potassium phosphate pH 6.9 10mg/ml protein in 25mM HEPES pH7.5, 50mM NaCl, 1mM TCEP. Mixed with compound (final concentration at 1mM) |
